RNA interference reveals the escape response mechanism of <i>Paramecium</i> to mechanical stimulation

In Paramecium, a mechanical stimulus applied to the posterior portion of cell causes transient increase in membrane permeability potassium ions, transiently rendering hyperpolarized state. Hyperpolarization Cyclic adenosine monophosphate (cAMP) concentration cilia, resulting fast-forward swimming cell. Schultz and coworkers (1992) reported that unique adenylate cyclase (AC)-coupled channel is involved reaction underlying this response, which known as “escape response.” However, AC responsible for remains be identified. Moreover, molecular linkage between mechanoreception activation has not been elucidated adequately. Currently, we can perform an efficient simple gene-knockdown technique Paramecium using RNA interference (RNAi). one several model organisms whole-genome sequences have elucidated. The RNAi whole genome derived from database (ParameciumDB) investigate types proteins elicit specific biological responses compare them with those other organisms. review, describe applications elucidating mechanism escape response identifying reaction. findings study highlight advantages ParameciumDB.